三种海洋浮游微藻的分子鉴定及培养条件研究     

徐娜  逄少军 《海洋科学》2012,36(10):13-25

采用分子生物学手段结合形态特征对海洋中生活着的浮游单细胞海藻进行准确无误的分类鉴定,正在成为一种普遍接受的方式。这样做的前提是首先需要从海水中分离得到纯藻株, 掌握它们的生活条件, 获得生物量, 并进而完成鉴定。作者对一株裸甲藻类似种和两株新分离的赤潮单细胞海藻进行了分离、培养和鉴定。核糖体18S RNA 基因(18S rDNA)和转录单元内间隔区(ITS)序列分析结果显示这三株藻分别为共生甲藻(Symbiodinium sp.)、锥状斯氏藻(Scrippsiella trochoidea)和赤潮异弯藻(Heterosigma akashiwo)。作者以广泛使用的光密度值A₆₈₀作为衡量藻体生物量变化的指标, 研究了这三种藻在不同培养条件下的生长。结果表明, 在批次培养中, 共生甲藻、锥状斯氏藻和赤潮异弯藻的生物量都在22℃达到最大, 分别为9.28×10⁵个/mL、7.9×10⁴个/mL 和4.75×10⁵个/mL; 强光和长时间光照促进生长, 但锥状斯氏藻在强光下更早进入衰退期; 三种藻都表现出较强耐受盐度变化的能力,在盐度为15～36 的培养液中生长良好; 营养实验表明, 使用882 μmol/L 的NO₃^- 和36.2 μmol/L 的PO₄^3-能够维持三种海藻的快速生长。  相似文献   

南黄海及长江口附近海域绿潮暴发前期微观繁殖体的动态变化   总被引：3，自引：2，他引：1  

方松  王宗灵  李艳  傅明珠  范士亮  王晓  张晓红 《海洋学报》2012,34(4):147-154

基于2011年3至5月对南黄海及长江口附近海域的水体与沉积物样品采集及实验室萌发培养实验,分析了绿潮暴发前期该海域微观繁殖体的时空分布特征。结果表明,在调查期间,南黄海水体中微观繁殖体空间分布趋势表现为近岸高远岸低,高值区主要集中在紫菜筏架养殖区附近海域,在122°30'E以东区域未发现有微观繁殖体存在。3,4,5月份该区域海水中平均微观繁殖体数分别为144 ,164和140株/dm3,最大值分别为278,426,和537株/dm3;南黄海沉积物中微观繁殖体数量分布范围介于19~127株/层,表层微观繁殖体数高于底层。长江口以北靠近江苏近岸区域有微观繁殖体存在,数量介于1~10株/dm3,31°N以南区域和河口内未发现有繁殖体分布,由此可排除黄海浒苔等绿藻微观繁殖体由长江水携带入海或来源于长江口南部海域。在春季绿潮暴发前开展该海域微观繁殖体的研究,可为查明绿潮发源地及早期发生发展过程提供基础资料,为预测和预警绿潮发生提供科技支撑。  相似文献   

Climate driven changes in the submerged macrophyte and phytoplankton community in a hard water lake     

《Limnologica》2015

We studied the changes in the submerged aquatic vegetation (SAV) and phytoplankton community in a hard water lake during different meteorological conditions. We hypothesized that variations in climatic conditions (precipitation and temperature) can influence the physicochemical parameters of water and, in turn, affect SAV and phytoplankton development. The investigations were performed in Lake Rogóźno (the West Polesie region, Eastern Poland) over 10 years from 2003 to 2013. The physicochemical parameters, the structure of macrophytes and the phytoplankton community in the dry (2003–2006, DP) and wet periods (2007–2013, WP) were analyzed. Between the dry and wet periods, the water color and the concentration of dissolved organic carbon (DOC) increased considerably, whereas water conductivity decreased. Other parameters (concentration of nutrients, water reaction and transparency) were comparable during both periods. When the precipitation and water level were low (DP), charophytes dominated the SAV and cyanobacteria dominated the phytoplankton community. After the precipitation and water level increased (WP), the charophyte population declined and the vascular plants and bryophytes dominated. Furthermore, flagellated algae belonging to the dinophytes and cryptophytes were the most numerous in the phytoplankton community. These changes in the SAV and phytoplankton were linked with the variations of physicochemical parameters determined by the total precipitation and mean air temperature in March.  相似文献   

秦皇岛入海河流的污染水平、排放特征及对褐潮暴发区生源要素的影响     

曹西华  俞志明  宋秀贤  袁涌铨  吴在兴  贺立燕 《海洋与湖沼》2017,48(5):970-981

为查明秦皇岛近海大面积褐潮连年暴发的成因,在2013年3—11月对该地区主要入海河流和沿岸褐潮暴发区的生源要素污染进行了连续调查研究。调查结果表明,所调查入海河流普遍为劣V类地表水,总氮(TN)超标严重,按照氮污染程度由高到低排列依次为大蒲河洋河戴河石河汤河东沙河。各河流中的碳、氮、磷、硅等污染物浓度在时间变化上没有统一规律。基于综合污染指数法的评价结果显示,大蒲河和洋河为重度污染,戴河和汤河从先前的轻度污染加重为中度污染。从污染物入海量上看,TN和化学需氧量(COD)是排放量最高的两种河源污染物,其中溶解态氮占TN的74.6%。在所调查河流中,洋河和大蒲河分别贡献了TN的38.2%和33.2%,同时大蒲河还贡献了75.8%的活性磷酸盐和37.7%的活性硅酸盐,而75.9%的COD来自汤河、洋河和大蒲河。秦皇岛河源污染物排放在时间上较为集中在6—9月的丰水期,但各河流单独的排放具有随机性,没有统一的季节性规律,表现出受人为调控影响明显的特点。秦皇岛沿岸褐潮暴发区的生源要素变化与河源污染物排放有显著性相关(P=0.05)。  相似文献   

长江口及其邻近海域有害藻华的发生情况、危害效应与演变趋势   总被引：3，自引：0，他引：3  

于仁成  张清春  孔凡洲  周正熙  陈振帆  赵越  耿慧霞  代丽  颜天  周名江 《海洋与湖沼》2017,48(6):1178-1186

长江口及其邻近海域是我国近海有害藻华高发区之一。自2000年以来,由东海原甲藻(Prorocentrum donghaiense)、米氏凯伦藻(Karenia mikimotoi)和亚历山大藻(Alexandrium spp.)等甲藻形成的大规模有害藻华连年暴发,对海水养殖业、人类健康和生态安全构成了严重威胁。以往调查和研究工作表明,长江口及其邻近海域藻华优势类群近年来已由硅藻逐渐转变为甲藻,同时,有害藻华原因种也表现出明显的年际变异特征和有毒有害藻种增多的态势,亟待解析其演变机理、趋势和驱动因素。长江口及其邻近海域受长江径流影响,富营养化问题突出,是大规模有害藻华形成的重要原因。同时,该海域也受到来自台湾东北部黑潮分支的影响,但对其与有害藻华的关系所知甚少。在中国科学院战略性先导科技专项"热带西太平洋海洋系统物质能量交换及其影响"支持下,针对这一问题开展了较为系统的调查和研究工作,本文对研究中得到的科学认识进行了初步总结。结果表明:东海黑潮分支能够到达长江口及其邻近海域赤潮区,影响该海域赤潮生物多样性状况;黑潮分支对长江口及其邻近海域甲藻赤潮发生过程具有重要调控作用;长江冲淡水和黑潮分支的共同作用决定了长江口及其邻近海域有害藻华的发生过程和演变趋势。在现有结果基础上进一步分析和讨论了未来长江口及其邻近海域有害藻华的研究方向,以期为进一步解析有害藻华演变趋势、提升有害藻华的监测和预警能力提供依据。  相似文献   

春季长江口富营养化水域浮游植物群集对冲淡水和上升流的响应   总被引：1，自引：1，他引：0  

宋书群  李照  李才文  俞志明 《海洋学报(英文版)》2017,36(12):101-110

A comprehensive study on the phytoplankton standing stocks, species composition and dominant species in the eutrophic Changjiang(Yangtze River) Estuary(CE) was conducted to reveal the response of phytoplankton assemblage to Changjiang Diluted Water(CDW) and upwelling in the spring. Phytoplankton presented peak standing stocks(13.03 μg/L of chlorophyll a, 984.5×103 cells/L of phytoplankton abundance) along the surface isohaline of 25. Sixty-six species in 41 genera of Bacillariophyta and 33 species in 19 genera of Pyrrophyta were identified, as well as 5 species in Chlorophyta and Chrysophyta. Karenia mikimotoi was the most dominant species, followed by Prorocentrum dentatum, Paralia sulcata, Pseudo-nitzschia delicatissima and Skeletonema costatum. A bloom of K. mikimotoi was observed in the stratified stations, where the water was characterized by low nitrate, low phosphate, low turbidity, and specific ranges of temperature(18–22 °C) and salinity(27–32). K.mikimotoi and P. dentatum accumulated densely in the upper layers along the isohaline of 25. S. costatum was distributed in the west of the isohaline of 20. Benthonic P. sulcata presented high abundance near the bottom,while spread upward at upwelling stations. CDW resulted in overt gradients of salinity, turbidity and nutritional condition, determining the spatial distribution of phytoplankton species. The restricted upwelling resulted in the upward transport of P. sulcata and exclusion of S. costatum, K. mikimotoi and P. dentatum. The results suggested that CDW and upwelling were of importance in regulating the structure and distribution of phytoplankton assemblage in the CE and the East China Sea.  相似文献   

Dynamics of phytoplankton associations in three reservoirs in northeastern Brazil assessed using Reynolds’ theory     

Ênio Wocyli Dantas  Maria do Carmo Bittencourt-OliveiraAriadne do Nascimento Moura 《Limnologica》2012,42(1):72-80

The aim of the present study was to evaluate the influence of seasonality on the behavior of phytoplankton associations in eutrophic reservoirs with different depths in northeastern Brazil. Five collections were carried out at each of the reservoirs at two depths (0.1 m and near the sediment) at three-month intervals in each season (dry and rainy). The phytoplankton samples were preserved in Lugol's solution and quantified under an inverted microscope for the determination of density values, which were subsequently converted to biomass values based on cellular biovolume and classified in phytoplankton associations. The following abiotic variables were analyzed: water temperature, dissolved oxygen, pH, turbidity, water transparency, total phosphorus, total dissolved phosphorus, orthophosphate and total nitrogen. The data were investigated using canonical correspondence analysis. The influence of seasonality on the dynamics of the phytoplankton community was lesser in the deeper reservoirs. Depth affected the behavior of the algal associations. Variation in light availability was a determinant of changes in the phytoplankton structure. Urosolenia and Anabaena associations were more abundant in shallow ecosystems with a larger eutrophic zone, whereas the Microcystis association was more related to deep ecosystems with adequate availability of nutrients. The distribution of Cyclotella, Geitlerinema, Planktothrix, Pseudanabaena and Cylindrospermopsis associations was different from that seen in subtropical regions and the substitution of these associations was related to a reduction in the eutrophic zone rather than the mixture zone.  相似文献   

Culture observation and molecular phylogenetic analysis on the blooming green alga Chaetomorpha valida (Cladophorales, Chlorophyta) from China     

邓蕴彦  汤晓荣  詹子锋  滕林宏  丁兰平  黄冰心 《中国海洋湖沼学报》2013,31(3):552-559

The marine green alga Chaetomorpha valida fouls aquaculture ponds along the coastal cities of Dalian and Rongcheng, China. Unialgal cultures were observed under a microscope to determine the developmental morphological characters of C. valida. Results reveal that gametophytic filaments often produce lateral branches under laboratory culture conditions, suggesting an atypical heteromorphic life cycle of C. valida between unbranched sporophytes and branched gametophytes, which differs from typical isomorphic alternation of Chaetomorpha species. The shape of the basal attachment cell, an important taxonomic character within the genus, was found variable depending on environmental conditions. The 18S rDNA and 28S rDNA regions were used to explore the phylogenetic affinity of the taxa. Inferred trees from 18S rDNA sequences revealed a close relationship between C. valida and Chaetomorpha moniligera. These results would enrich information in general biology and morphological plasticity of C. valida and provided a basis for future identification of green tide forming algae.  相似文献   

Comparing long term sediment records to current biological quality element data - Implications for bioassessment and management of a eutrophic lake     

Tommi Kauppila  Antti Kanninen  Matias ViitasaloJohanna Räsänen  Kristian MeissnerJukka Mattila 《Limnologica》2012,42(1):19-30

Defining reference conditions for lakes situated in areas of human settlement and agriculture is rarely straightforward, and is especially difficult within easily eroding and nutrient rich watersheds. We used diatoms, cyanobaterial akinetes, remains of green algae and chironomid head capsules from sediment samples of Lake Kirmanjärvi, Finland, to assess its deviation from the initial ecological status. These site-specific records of change were compared to current type-specific ecological status assessment. All paleolimnological data indicated deviation from natural conditions and mirrored the current, monitoring-based assessment of “moderate” ecological lake status. However, the sediment data showed that the lake should be re-typified as a naturally eutrophic lake. Sediment records as well as current monitoring data indicated temporary improvement in water quality in response to extensive fish manipulation. Our results suggest that paleolimnological records can be used to derive site-specific reference conditions and that extensive restoration efforts can result in gradual, observable improvements of water quality and ecological status.  相似文献   

Recurrence of the world’s largest green-tide in 2009 in Yellow Sea, China: Porphyra yezoensis aquaculture rafts confirmed as nursery for macroalgal blooms     

Dongyan Liu  John K. Keesing  Zhijun Dong  Baoping Di  Peter Fearns 《Marine pollution bulletin》2010,60(9):1423-1432

In the summer of 2008, the world’s largest green-tide occurred in the Yellow Sea, China. The hypothesized cause was the expansion of Porphyra yezoensis aquaculture along the Jiangsu coastline and the re-occurrence of a green-tide in 2009 was predicted. In this study, satellite and field images showed the formation of the June 2009 green-tide which again originated from the Jiangsu coast. The responsible species, its source and biomass accumulation were studied to support the previous hypothesis. Morphological and phylogenetic analysis demonstrated the homology of Ulva prolifera in the 2008 green-tide with the U. prolifera from P. yezoensis aquaculture rafts. About 91-505 kg/ha of U. prolifera was attached to the P. yezoensis aquaculture rafts and a total biomass of 4956 tonnes was estimated during the harvesting of P. yezoensis. This is sufficient to seed a bloom when they are dislodged from the rafts as a result of harvesting practices.  相似文献